蛋白质体分析实验室简介
篇一:chen,chih,ying芝芝
蛋白質體分析實驗室簡介
一、 個人基本資料
姓 名：徐睿良 (Jue-Liang Hsu)
電 話：08-7703202 # 5197
電子郵件：jlhsu@mail.npust.edu.tw
學 歷：台灣大學化學博士
專 長：1. 蛋白質體學 2. 質譜分析 3. 有機合成
二、 實驗室研究方向
本實驗室的主要研究方向在於建立蛋白質體相關的分析平台與開發生醫領域相關的分析載具。在蛋白質體相關的分析平台方面，我們希望能開發創新的質譜分析技術及建立蛋白表現分析、轉譯後蛋白分析及蛋白—蛋白作用分析等技術平台，並進一步地應用在蛋白質體研究與生醫樣品的蛋白質檢測。同時，我們也希望將這些分析或純化的技術整合於奈米或微米載具上，開發出高效能分析檢驗產品，以利生醫研究的發展。
三、發表著作
A. 期刊論文
1. Ho, Fong-Ku Shi, Ing-Feng Chang, “Functional phosphoproteomic profiling of
phosphorylation sites in membrane fractions of salt-stressed Arabidopsis thaliana”, accepted for publication in Proteome Science. (SCI; Categories: Biochemical Research methods; Ranking: 26/65; IF: 2.537)
2. Shu-Hui Chen, Yi-Wen Wang, Jing-Jing Chuang, Hung-Wen Tsai, Chi-Wu Chiang, Chung-Ta Lee, Fang-Chih Chang, Hsiao-Sheng Liu, Nan-Haw Chow, “Nucleophosmin in the Pathogenesis of Arsenic-related Bladder Carcinogenesis Revealed by Quantitative Proteomics”, accepted for publication in Toxicology and Applied Pharmacology. (SCI;
Categories: Pharmacology & Pharmacy; Ranking: 55/219; IF: 3.364)
3. Sheng-Yu. Huang, Chien-Hsien. Wen, Ding-Tzai Li,Chen, Fong-Ku Shi, and Yueh Yi Lin, “Assignment of Disulfide-Linked Peptides Using Automatic a1 Ion Recognition.” Anal. Chem. 2008, 80, 9135-9140. (SCI)
4. Shu-Hui Chen, , Fong-Sian Lin. “Fluorescein As a Versatile Tag for Enhanced Selectivity in Analyzing Cysteine-containing Proteins/Peptides Using Mass Spectrometry”, Anal. Chem. 2008, 80, 5251?5259. (SCI)
5. Po-Tsun Shen, “Dimethyl Isotope-Coded Affinity Selection for the Analysis of Free and Blocked N-Termini of Proteins Using LC-MS/MS”, Anal. Chem. 2007, 79, 9520?9530. (SCI)
6. Shu-Hui Chen, Ding-Tzai Li, and Fong-Ku Shi. “Enhanced a1 Fragmentation for Dimethylated Proteins and Its Applications for N-terminal Identification and Comparative Protein Quantitation”, J. Proteome Res. 2007, 6, 2376?2383. (SCI)
7. Sheng-Yu Huang, and Shu-Hui Chen. “Dimethyl Multiplexed Labeling Combined with Microcolumn Separation and MS Analysis for Time Course Study in Proteomics”, Electrophoresis 2006, 27, 3652?3660. (SCI)chen,chih,ying芝芝。
8. Sheng-Yu Huang, Chin-Jen Wu, Mei-Ling Tsai, and Shu-Hui Chen. “Quantitation of protein phosphorylation in pregnant rat uteri using stable isotope dimethyl labeling coupled with IMAC ”, Proteomics 2006, 6, 1722?1734. (SCI)
9. “Recent Progress in Quantitative Proteomics Using Stable Isotope Labeling, Multidimensional Liquid Chromatography and Mass Spectrometry”, Current Proteomics 2005, 2, 287?302. (SCI)
10. Sheng-Yu Huang, and Shu-Hui Chen. “Two-step Immobilized Metal Affinity Chromatography (IMAC) for Phosphoproteomics Using Mass Spectrometry”, J. Chin. Chem. Soc., Taipei, 2005, 52, 765-772. (SCI)
11. Sheng-Yu Huang, Jen-Taie Shiea, Wen-Ying Huang, and Shu-Hui Chen. “Beyond Quantitative Proteomics: Signal enhancement of the a1 ion as a mass tag for peptide sequencing using dimethyl labeling”, J. Proteome Res. 2005,
4, 101-108. (SCI)
12. Wu, Fong-Ku Shi, Shu-Hui Chen, “Photopolymerized micro-tips for sample preparation in proteomic analysis” Electrophoresis 2004, 25, 3840-3847. (SCI)
13. Sheng-Yu Huang, , Nicholas A. Morrice, Chin-Jen Wu and Shu-Hui Chen. “A convenient method to extract MALDI-MS spectra from phosphate-containing peptide mixtures” Proteomics 2004, 4, 1935-1938. (SCI)
14. “Stable-isotope dimethyl labeling for quantitative proteomics” Anal. Chem. 2003, 75, 6843-6852. (SCI)chen,chih,ying芝芝。
15. Hui Chen “ Stable isotope labeling coupled with LC/MS technique for quantitative proteomics” (in Chinese), CHEMISTRY（The Chinese Chem.Soc, Taipei）, 2003, 61, 257-266
16. Ming-Yi Chen, , Jiun-Jie Shie and Jim-Min Fang. “Direct
oxidative amidation of aldoses by iodine in ammonia water”. J. Chin. Chem. Soc., Taipei, 2003, 50, 129-133. (SCI)
17. “Stereoselective synthesis of ?-lactones from 5-oxoalkanals via one-pot sequential acetalization, Tishchenko reaction, and lactonization by cooperative catalysis of samarium ion and mercaptan”. J. Org. Chem. 2001, 66, 8573-8584. (SCI)
18. Sanjay Talukdar, “Direct transformation of aldehydes to nitriles using iodine in ammonia water”.
Tetrahedron Lett. 2001, 42, 1103-1105. (SCI)
19. Jim-Min Fang, Ming-Yi Chen, Jiann-Shyng Shiue, Ling Lu and “Samarium diiodide-mediated asymmetric reactions of 8-phenylmenthyl esters”. Tetrahedron Lett. 2000, 41, 4633-4636. (SCI)
20. “Cooperative catalysis of samarium diiodide and mercaptan in a stereoselective one-pot transformation of 5-oxopentanals into delta-lactones”. Organic Lett. 1999, 1, 1989-1991. (SCI)
21. Guo-Hsian Chen, , Wen-Bin Yang, Jim-Min Fang, Gene-Hsiang Lee, Yi-Hung Liu, and Yu Wang. “Preparation of chiral phosphorus (V) reagents and their uses with borane in the enantioselective reduction of ketones”. J. Chin. Chem. Soc. Taipei, 1999, 46, 797-810. (SCI)
B. 研討會論文
1. “Development of MS-based Approach for Large-scale Membrane Protein Expression Profiling and Its Application in Comprehensive Study for Membrane
Protein Expression of Gallic Acid-treated A375 Cells” in “International Symposium on Innovative Agricultural Biotechnology (2009) ” in Pintung, Taiwan by Yao-Chi Tsai and 2. “Enrichment and Analysis of Phosphopeptides Shaved from Membrane Fraction of Salt-stressed Arabidopsis” in “2009 TPS International Proteomics Conference and 5th AOHUPO MPI Workshop (2009) in Taipei, Taiwan” by Lan-Yu Wang, Shu-Ying Wang, Kuo-Chieh Ho, Ing-Feng Chang.
3. “Enrichment of glycopeptides from complex mixture by using HILIC magnetic beads and its application on simple and robust protein N-glycosylation sites determination” in “2008 Taiwan-Japen Proteomics Symposium (Taipei)” by and Fong-Ku Shi.
4. “Directly identify protein N-terminal residues by mass spectrometry and its potential applications in protein-level comparative proteomics” in “55th ASMS Conference on Mass Spectrometry (2007) in Indianapolis, Indiana (USA)” by Ding-Tzai Li, and Fong-Ku Shi.
5. “Selective Detection of Glycoproteins in Polyacrylamide Gels Using an Rh-Hydrazide Fluorescent Dye” in Taiwan Proteomics Society International Conference, 2007, by Ya-Ting Lu, Ding-Tzai Li, and Fong-Ku Shi.
6. “On-Target Detection of Phosphorylated Peptides Using Zr(IV)Nitrilotriacetate Complexes Immobilized on a MALDI Plate” in Taiwan Proteomics Society International Conference, 2007 by and Fong-Ku Shi.
7. “An efficient and convenient phosphoprotein purification column for the enrichment of phosphoproteins from protein mixture” in “Chinese chemical society meeting 2006, Taipei”by
8. “Chemical derivitization-assisted top-down approach to directly identify protein N-terminal residues and its applications in protein-level comparative proteomics” in “Chinese chemical society meeting 2006, Taipei”by Ding-Tzai Li, and Fong-Ku Shi.
9. “Fabrications of functional MALDI plates and their applications in on-target sample preparations for MALDI-TOF analysis” in “Chinese chemical society meeting 2006, Taipei”by and Lan-Yu Wang.
10. “Stable-Isotope based Multiplex Labeling Coupled with LC-MS/MS Using in Protein Expression Profiling: Potential Application in Study of Time Course or Dosage Effect for Protein Expression” in “HUPO 3rd ANNUAL WORLD CONGRESS, Bejing (China) 2004”by , Sheng-Yu Huang, and Shu-Hui Chen.
11. “Beyond Quantitative Proteomics: Signal Enhancement of the a1 ion as a Mass Tag for Peptide Sequencing Using Dimethyl Labeling” in “2004 International Conference of Advanced Biotechnology Dialogue between Proteomics and Biotechnology, Tainan” by Shu-Hui Chen.
12. “Novel Stable-isotope labeling for Quantitative Proteomics and its Application for Protein Expression Profiling”by , Sheng-Yu Huang, and Shu-Hui Chen.“HUPO 2nd Annual & IUBMB XIX Joint World Congress, Montreal (Canada) 2003”
13. “Novel FluoresceiIn Affinity Chromatography for Protein Characterization Using Mass Spectrometry”by and Shu-Hui Chen in“HUPO 2nd Annual & IUBMB XIX Joint World Congress, Montreal (Canada) 2003”
14. “Two-step immobilized metal affinity chromatography (IMAC) for the analysis of phosphoproteins using mass spectrometry” by Sheng-Yu Huang, and Shu-Hui Chen in “ISPPP, Florida (USA) 2003”.
C. 專利
1.“用於濃縮純化生化樣品之拋棄式吸管及其製造方法”（台灣專利：
200500272），陳淑慧、周孟寬、徐睿良及石峰鵠。
2.“Reagent kit of global analysis for protein expression and method for qualitative and quantitative proteomic analysis using the same” (US Patent: 7338806) by Shu-Hui Chen, , Sheng-Yu Huang.
D. 其他著作
1. 化學—結構與動力（台灣西書出版）：Spencer原著/徐睿良 譯
四、承接計畫
论文发表
篇二:chen,chih,ying芝芝
論文發表
自2005年迄今，共發表國際高分子期刊論文 30 篇，詳細的作者、篇名、期刊名，以及頁數均列於下表：
學術活動
自2005年迄今，所有應邀參與之學術活動的日期及地點均詳列於下列表中： 日期
20050901
200509192005092620051004200510152005110320051107活動及地點chen,chih,ying芝芝。
國科會聘任為群體策略規劃材料領域召集人,主持國家材料
領域之未來發展整體規劃。
Keynote speaker and section chair International Polymer
Materials Engineering Conference, 上海 [POSS Related Nanocomposites]
Plenary speaker East Asia Symposium on Functional Dyes
and Advanced Materials,台北 [POSS Related Nanocomposites]
台北科技大學分子研究所演講 [高分子領域為理工科學生
之未來選擇]
Invited speaker and section chair Advance in Polymer LC
and Supramolecular Ordered Systems,四川成都 [Synthesis of the Organic/Inorganic Hybrid Star Polymers and Their Inclusion Complexes with Cyclodextrines]
桃園南亞技術學院化材學院演講 [介紹高分子領域為理工科
學生未來之選擇]
拜訪廣州中山大學高分子工程系做學術交流及演講[Polymer
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